ELISA Kit DIY Materials for Albumin (ALB) Canis familiaris; Canine (Dog) Assay Kit DIY Materials

Serum albumin

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ug/mL
  • Applicable Sensitivity0.45ug/mL
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  • ELISA Kit DIY Materials for Albumin (ALB) Packages (Simulation)
  • ELISA Kit DIY Materials for Albumin (ALB) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Albumin (ALB). No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical FormationPubMed: 22701728
  • Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pupsOnlinelibrary: liv.12219
  • Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in micePubmed: 24273183
  • Increased Circulating Levels of Vitamin D Binding Protein in MS PatientsPubmed:25590278
  • The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN micePubMed: 26346164
  • Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human StudyPubmed:26934615
  • 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineeringart00007
  • Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy.pubmed:29246788
  • Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung InjuryPubmed:30014231
  • Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspringPubmed:29582727
  • Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human studyPubmed:29384875
  • Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failurePubmed: 30463600
  • Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV InfectionPubmed: 30963688
  • Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarizationPubmed: 31146107
  • Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in micePubmed: 31256934
  • A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationPubmed: 31447572
  • Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activityPubmed: 31580898
  • Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumoniaPubmed: 31655595
  • Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathwayPubmed: 31918060
  • Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathwayPubmed: 32908237
  • Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese RatsPubmed: 32399849
  • Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats
  • Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats
  • A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate
  • cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling33721834
  • A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle
  • Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway34807349
  • GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
  • Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions34914377
  • Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in micePubmed:35708145
  • Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy

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