ELISA Kit DIY Materials for Allopregnanolone (AP)
THP; 3a,5a-Tetrahydro Progesterone
- UOM
- FOB US$ 1,454.00 US$ 2,424.00 US$ 4,363.00 US$ 8,484.00 US$ 14,544.00
- Quantity
Overview
Properties
- Product No.KSB963Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayEndocrinologyReproductive scienceGenetic scienceNutrition metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ng/mL
- Applicable Sensitivity0.55ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Allopregnanolone (AP). No significant cross-reactivity or interference between Allopregnanolone (AP) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Early repeated administration of progesterone improves the recovery of neuropathic pain and modulates spinal 18 kDa-translocator protein (TSPO) expressionPubmed:24607808
- Isolation Rearing Reduces Neuronal Excitability in Dentate Gyrus Granule Cells of Adolescent C57BL/6J Mice: Role of GABAergic Tonic Currents and Neurosteroidspubmed:27378855
- Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and …Pubmed: 32182704
- A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡33862428