ELISA Kit DIY Materials for Alpha-1-Acid Glycoprotein (a1AGP) Homo sapiens (Human) Assay Kit DIY Materials

AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range6.25-400ng/mL
  • Applicable Sensitivity2.76ng/mL
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  • ELISA Kit DIY Materials for Alpha-1-Acid Glycoprotein (a1AGP) Packages (Simulation)
  • ELISA Kit DIY Materials for Alpha-1-Acid Glycoprotein (a1AGP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-1-Acid Glycoprotein (a1AGP). No significant cross-reactivity or interference between Alpha-1-Acid Glycoprotein (a1AGP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In VitroPubmed:Pmc4219990
  • Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitroPubmed:25123565
  • Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical studyPubmed:25652121
  • Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot StudyPubMed: 25955845
  • S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabinepmc:PMC4810364
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858
  • An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattlepubmed:28667317
  • Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.pubmed:28927749
  • Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndromePubmed: 30213594
  • Proteome-wide characterization and biomarker identification of intracranial aneurysm
  • Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancerPubmed: 31482685
  • Effects of vaccination on acute-phase protein response in broiler chickenPubmed: 32191767
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME)

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