ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH) Homo sapiens (Human) Assay Kit DIY Materials

α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.5-10000pg/mL
  • Applicable Sensitivity46.5pg/mL
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  • ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH) Packages (Simulation)
  • ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Melanocyte Stimulating Hormone (aMSH). No significant cross-reactivity or interference between Alpha-Melanocyte Stimulating Hormone (aMSH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone.Pubmed: 23341471
  • Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by lightPubMed: 26582755
  • Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in MicePubmed:27080257
  • Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by lightPubmed:26582755
  • Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives.pubmed:28794470
  • Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone ResponsesPubmed:29681516
  • Epigenetic alterations of the POMC promoter in tobacco dependencePubmed:29871818
  • Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factorPubmed:29685637
  • Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol DependencePubmed: 31117084
  • Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathwayPubmed: 32892076
  • Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …Pubmed:35098494

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