ELISA Kit DIY Materials for Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)
SCYE1; EMAP II; EMAP2; p43; Small Inducible Cytokine Subfamily E,Member 1; Endothelial monocyte-activating polypeptide II; Multisynthase complex auxiliary component p43
- UOM
- FOB US$ 1,386.00 US$ 2,310.00 US$ 4,158.00 US$ 8,085.00 US$ 13,860.00
- Quantity
Overview
Properties
- Product No.KSL761Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryEnzyme & Kinase
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range125-8000pg/mL
- Applicable Sensitivity46pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). No significant cross-reactivity or interference between Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Endothelial monocyte activating polypeptide II in children and adolescents with type 1 diabetes mellitus: Relation to micro-vascular complicationsPubMed: 26142824
- Serum Aminoacyl-tRNA Synthetase-Interacting Multifunctional Protein-1 Can Predict Severe Antineutrophil Cytoplasmic Antibody-Associated Vasculitis: A Pilot …