ELISA Kit DIY Materials for Annexin V (ANXA5)
ANX5; ENX2; PP4; PAP-I; CBP-I; Anchorin CII; Calphobindin I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; Thromboplastin inhibitor; Vascular anticoagulant-alpha
- UOM
- FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
- Quantity
Overview
Properties
- Product No.KSA259Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryApoptosisHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1000pg/mL
- Applicable Sensitivity6.4pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Annexin A5 (ANXA5). No significant cross-reactivity or interference between Annexin A5 (ANXA5) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Markers of apoptotic dysfunctions in schizophreniaPubmed: 24466757
- Involvement of Anomalous Apoptosis in Impairments to Synaptic Plasticity in Post-Traumatic Stress DisorderSpringer: Source
- Collectins, C3 complement protein, annexin V and C-reactive protein in acute ischemic stroke: interrelation and implication to upregulated apoptosis and inflammationSmartscitech: Source
- Annexin A5 Protein as a Potential Biomarker for the Diagnosis of AsthmaPubmed: 30182154