ELISA Kit DIY Materials for Antidiuretic Hormone (ADH)
ARVP; AVP; VP; Arginine Vasopressin; Arg-Vasopressin; Antidiuretic Hormone; Diabetes Insipidus; Neurohypophyseal; Argipressin
- UOM
- FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
- Quantity
Overview
Properties
- Product No.KSB139Hu11
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionMetabolic pathwayEndocrinologyNeuro scienceHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range12.35-1000pg/mL
- Applicable Sensitivity4.91pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Antidiuretic Hormone (ADH). No significant cross-reactivity or interference between Antidiuretic Hormone (ADH) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure RatsPubmed:29684507
- Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient micePubmed:29363324
- Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema