ELISA Kit DIY Materials for Apelin (APLN)

XNPEP2; APEL; APJ endogenous ligand

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,368.00 US$ 2,280.00 US$ 4,104.00 US$ 7,980.00 US$ 13,680.00
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Overview

Properties

Product No.KSD066Ra11
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategoryEndocrinology

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range12.35-1000pg/mL
Applicable Sensitivity4.84pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Apelin (APLN). No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

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ELISA / CLIA Experiment Service

Increment services

Citations

Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced HypertensionInformahealthcare: Source
Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat.Academicjournals: Source
Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension RatsZjrms: Source
CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytesPubmed:25168658
TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic micePubMed: 26500845
Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design.pubmed:28170279
Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise trainingPubmed: 31290696
Serum Apelin and Galactin-3 in Preeclampsia in IraqPubmed: 32538210
The Potential Role of Chemerin, Lipocalin 2, and Apelin in the Diagnosis and Pathophysiology of Gestational Diabetes Mellitus