ELISA Kit DIY Materials for Apolipoprotein A1 (APOA1) Capra hircus; Caprine (Goat) Assay Kit DIY Materials

Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range6.25-400ng/mL
  • Applicable Sensitivity2.73ng/mL
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  • ELISA Kit DIY Materials for Apolipoprotein A1 (APOA1) Packages (Simulation)
  • ELISA Kit DIY Materials for Apolipoprotein A1 (APOA1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1). No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Endurance training enhances ABCA1 expression in rat small intestinePubMed: 19629515
  • Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. Wrestling: source
  • Treadmill exercise enhances ABCA1 expression in rat liverScienceDirect: S0006291X0701488X
  • The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1Idosi: source
  • Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant propertiesPubMed: 23328279
  • The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry studyPubMed: 22458349
  • MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? MicePubMed: PMC3540673
  • Apolipoprotein AI Is a Potential Mediator of Remote Ischemic PreconditioningPlosone: Source
  • DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levelsPubmed: 23920041
  • Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice Pubmed: 24684302
  • Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemiaSciencedirect:S2214426915000026
  • Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapyPubMed: 26253619
  • Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolismPubMed: 26515253
  • Efficacy of tomato concentrates in mouse models of dyslipidemia and cancerPubMed: 26171234
  • Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndromePubMed: 26351173
  • Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesisPubMed: 26279300
  • Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesisPubmed:26279300
  • Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis.pubmed:28378321
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858
  • High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signalingpubmed:27866837
  • Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice10.15616/BSL.2017.23.2.80
  • Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modificationabs:10.1021
  • Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivoPubmed:29580541
  • Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humansPubmed:29124773
  • A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damagePubmed:29654869
  • The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?…Pubmed: 30768981
  • High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male ratsPubmed: 31718266
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and CatsPubmed: 32880234
  • GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
  • Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal StudyPubmed:35429258
  • Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptorPubmed:35660638
  • Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages …

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