ELISA Kit DIY Materials for Aquaporin 4 (AQP4)

HMIWC2; MIWC; WCH4; Mercurial-insensitive water channel

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
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Overview

Properties

Product No.KSA582Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategorySignal transduction Metabolic pathway Infection immunity Neuro science

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range62.5-4000pg/mL
Applicable Sensitivity23.3pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Aquaporin 4 (AQP4). No significant cross-reactivity or interference between Aquaporin 4 (AQP4) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patientsScienceDirect: S0304394011012341
Astrocytic damage is far more severe than demyelination in NMONeurology: 208
Aquaporin-4 expression in the cerebrospinal fluid in congenital human hydrocephalusPubMed: PMC3651869
Melanocortin MC4 receptor agonists alleviate brain damage in abdominal compartment syndrome in the ratPubMed: 25616531
Novel effect of Daflon and low-dose γ-radiation in modulation of thioacetamide-induced hepatic encephalopathy in male albino ratsPubmed:26987350
Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertensionPubmed:26853804
Cerebrospinal fluid biomarkers of infantile congenital hydrocephalus.pubmed:28212403
Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain InjuryPubmed: 31441374
Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease33995926
Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus34597351