ELISA Kit DIY Materials for Asymmetrical Dimethylarginine (ADMA) Pan-species (General) Assay Kit DIY Materials

N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range24.69-2,000ng/mL
  • Applicable Sensitivity8.98ng/mL
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  • ELISA Kit DIY Materials for Asymmetrical Dimethylarginine (ADMA) Packages (Simulation)
  • ELISA Kit DIY Materials for Asymmetrical Dimethylarginine (ADMA) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Asymmetrical Dimethylarginine (ADMA). No significant cross-reactivity or interference between Asymmetrical Dimethylarginine (ADMA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in HighlandersPubMed: PMC3439466
  • Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats.Pubmed: 23834755
  • Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats.Pubmed:25475507
  • Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patientsPubmed:25550968
  • Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPDPubMed: 26076870
  • Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive ratsPubMed: 26375520
  • Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in ratsPubMed: 25475507
  • Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Studypubmed:27677852
  • Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease.pubmed:27884754
  • Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans.pubmed:26840628
  • Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA).pubmed:29150732
  • A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK SignalingPubmed:29523851
  • Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territoriesPubmed:29605639
  • Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newbornsDoi: 10.1016/j.numecd.2018.09.002
  • Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsPubmed: 30454888
  • Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
  • Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathwayPubmed: 30673277
  • Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats
  • The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT CellsPubmed: 32104540
  • Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in …Pubmed: 31746378
  • Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis PatientsPubmed: 33163233
  • The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical TrialPubmed: 32344735
  • Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls33463628
  • Non-functioning adrenal incidentalomas may increase toxic metabolites34255169
  • Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna34959638
  • HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancerPubmed:35644470

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