ELISA Kit DIY Materials for Beta-Crosslaps (bCTx)

b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
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Overview

Properties

Product No.KSA892Ra11
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryBone metabolism

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range98.8-8000pg/mL
Applicable Sensitivity37.5pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Beta-Crosslaps (bCTx). No significant cross-reactivity or interference between Beta-Crosslaps (bCTx) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Effects of carbamazepine on serum parathormone, 25-hydroxyvitamin D, bone specific alkaline phosphatase, C-telopeptide, and osteocalcin levels in healthy ratsPubMed: 23198939
Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesionsPubmed:Pmc3946460
Elevated serum soluble CD200 and CD200R as surrogate markers of bone loss under bed rest conditionsPubmed:24333170
Effect of Urocortin on strength and microarchitecture of osteopenic rat femurPubmed:24633537
Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D DeficiencyPubmed:29897564
Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats10.1016:j.jff.2018.04.048
Biomechanical and histological analyses of the fracture healing process after direct or prolonged reductionPubmed: 30180907
FGF23 and primary hyperparathyroidism: is there a link?Pubmed: 32598019
血清骨膜素水平与老年髋部骨折患者骨密度相关性研究.