ELISA Kit DIY Materials for Beta-Endorphin (bEP)
b-EP
- UOM
- FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
- Quantity
Overview
Properties
- Product No.KSA806Mu11
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionEndocrinologyNeuro scienceHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range12.35-1000pg/mL
- Applicable Sensitivity4.77pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP). No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Endogenous μ-opioid peptides modulate immune response towards malignant melanomaPublish: RD10279
- Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave SpringerLink: tv015l3126833315
- Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophageIjpsi: Source
- Evaluation of immune parameters in chronic migraine with medication overusePubmed:24867859
- Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria.pubmed:28413714
- Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection propertiesDOI: 10.1111/jocd.12349
- Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trialPubmed:29512230
- КОРРЕКЦИЯ ЦИТОКИНОВОГО И ГОРМОНАЛЬНОГО ДИСБАЛАНСА ПРИ ЛЕЧЕНИИ СТАБИЛЬНОЙ СТЕНОКАРДИИ НАПРЯЖЕНИЯ10.25207:1608-6228-2018-25-3-51-55
- Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria
- Blood dopamine level enhanced by caffeine in men after treadmill runningPubmed: 31793465
- Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in …Pubmed: 31553937
- Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissivesPubmed: 32044259
- Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-InjuryPubmed: 32961416
- Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot studyPubmed: 32855024
- Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats34309681
- Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …