ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB)
OHB; BHBA; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid; β-Hydroxybutyric acid
- UOM
- FOB US$ 1,454.00 US$ 2,424.00 US$ 4,363.00 US$ 8,484.00 US$ 14,544.00
- Quantity
Overview
Properties
- Product No.KSB022Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionMetabolic pathwayEndocrinology
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ug/mL
- Applicable Sensitivity0.48ug/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Beta-Hydroxybutyric Acid (bHB). No significant cross-reactivity or interference between Beta-Hydroxybutyric Acid (bHB) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expressionEndocrine:Source
- A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitroPubMed: 25846235
- Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methioninepubmed:27501393
- Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes
- Temporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows