ELISA Kit DIY Materials for Beta-Site APP Cleaving Enzyme 1 (bACE1) 
ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2
- UOM
- FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
- Quantity
Overview
Properties
- Product No.KSA718Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryNeuro science
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, cerebrospinal fluid and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.250-16ng/mL
- Applicable Sensitivity0.11ng/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Beta-Site APP Cleaving Enzyme 1 (bACE1). No significant cross-reactivity or interference between Beta-Site APP Cleaving Enzyme 1 (bACE1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Comparative study of the effects of phosphatidylcholine rich in DHA and EPA on Alzheimer’s disease and the possible mechanisms in CHO-APP/PS1 cells and SAMP8 micepubmed:29292421
- EPA enriched ethanolamine plasmalogens significantly improve cognition of Alzheimer's disease mouse model by suppressing β-amyloid generation10.1016/j.jff.2017.12.016
- Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat dietPubmed:29986309
- Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
- Effects of Alcohol Intake on Cognitive Function and ¦Â-amyloid Protein in APP/PS1 Transgenic Mice33737111