ELISA Kit DIY Materials for Bone Morphogenetic Protein 4 (BMP4)
BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B
- UOM
- FOB US$ 1,057.00 US$ 1,761.00 US$ 3,170.00 US$ 6,164.00 US$ 10,566.00
- Quantity
Overview
Properties
- Product No.KSA014Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryDevelopmental scienceBone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.070ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4). No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling PathwayPubMed: 26681207
- Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serumPubmed:26945137
- Bone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progressionPubmed: 30972973
- The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese …34417839