ELISA Kit DIY Materials for Brain Natriuretic Peptide (BNP) Rattus norvegicus (Rat) Assay Kit DIY Materials

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range4.94-400pg/mL
  • Applicable Sensitivity1.82pg/mL
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  • ELISA Kit DIY Materials for Brain Natriuretic Peptide (BNP) Packages (Simulation)
  • ELISA Kit DIY Materials for Brain Natriuretic Peptide (BNP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Brain Natriuretic Peptide (BNP). No significant cross-reactivity or interference between Brain Natriuretic Peptide (BNP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sicknessAPS: source
  • Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation.PubMed: 21297953
  • Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in RatsPubmed: 24055824
  • ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHYOnlinejacc:Source
  • Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing?Pubmed:24679871
  • Cardioprotective effects of baicalein on heart failure via modulation of Ca2PubMed: 26706290
  • Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot studyPublication: 5950761
  • Renal Sympathetic Denervation Improves Cardiac DysfunctionPubMed: 25804096
  • Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitroPubmed:26706290
  • Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathwayPubmed:26970569
  • Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction.pubmed:27779336
  • The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic …pubmed:27652141
  • Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure.pubmed:27555054
  • Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathypubmed:27693530
  • Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP.pubmed:28091624
  • Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failurepubmed:28627670
  • Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathypubmed:29240788
  • Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955
  • Metformin attenuated endotoxin-induced acute myocarditis via activating AMPKpubmed:28410530
  • Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy.pubmed:29063791
  • Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
  • An essential role for Wnt/β-catenin signaling in mediating hypertensive heart diseasePubmed:29895976
  • Cardioprotective effect of substance P in a porcine model of acute myocardial infarctionPubmed:29884290
  • High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failurePubmed:30072155
  • Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy SubjectsPubmed:29977313
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart DiseasePubmed:29392348
  • Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosisPubmed:29321061
  • 和肽素水平对大鼠心肾综合征的早期预测价值filename:ZBLS201802013
  • Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
  • Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar ratsPubmed: 30426860
  • Van Veterinary Journal
  • Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndromePubmed: 30770217
  • Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytesPubmed: 30982981
  • Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
  • Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitorPubmed: 32570118
  • Empagliflozin improves left ventricular diastolic function of db/db micePubmed: 32353614
  • Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF¦Â1/Smad ¡­34116674
  • Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis
  • κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a34373768
  • Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues34633055
  • Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model34452454
  • Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stressPubmed:35218795

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