ELISA Kit DIY Materials for E-cadherin 
CDHE; CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule
- UOM
- FOB US$ 1,160.00 US$ 1,933.00 US$ 3,479.00 US$ 6,766.00 US$ 11,598.00
- Quantity
Overview
Properties
- Product No.KSA017Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryCD & Adhesion moleculeTumor immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, urine, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range62.5-4000pg/mL
- Applicable Sensitivity25.1pg/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Cadherin, Epithelial (CDHE). No significant cross-reactivity or interference between Cadherin, Epithelial (CDHE) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Novel method for proliferation of oral keratinocyte stem cellsPubmed: 24329011
- Modulation of the Dual‐Faced Effects of miR‐141 with Chitosan/miR‐141 Nanoplexes in Breast Cancer CellsPubmed: 31389101
- BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherinPubmed: 31957537
- Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy …Pubmed: 32342301
- Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …