ELISA Kit DIY Materials for Calcitonin (CT) Mus musculus (Mouse) Assay Kit DIY Materials

CALCA; CALC1; KC; CCP; PDN-21; Katacalcin; Calcitonin carboxyl-terminal peptide

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.81pg/mL
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  • ELISA Kit DIY Materials for Calcitonin (CT) Packages (Simulation)
  • ELISA Kit DIY Materials for Calcitonin (CT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Calcitonin (CT). No significant cross-reactivity or interference between Calcitonin (CT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effect of consumption of fatty acids, calcium, Vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in ratsNiscair: 13737
  • The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in ratPubMed: 22782709
  • Interleukin-2 and Lanreotide in the Treatment of Medullary Thyroid Cancer: In Vitro and In Vivo StudiesPubmed: 23884781
  • Effect of whole body vibration on healthy rat plasma parametersEbscohost: Source
  • Effect of consumption of fatty acids, nutrients and regular physical activity on bone mechanical properties in ratsPubmed:22439438
  • Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies.pubmed:27448535
  • Cell-matrix signals specify bone endothelial cells during developmental osteogenesis.pubmed:28218908
  • Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat10.22192/ijarbs.2017.04.04.018
  • p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathwayPubmed: 32215984
  • Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmiaPubmed: 33149301
  • Copeptin in fluid disorders and stressPubmed:35143773

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