ELISA Kit DIY Materials for Casein Alpha (CSN1)
CASa; CSN1; CSN1S1; Casein Alpha S1; Caseinate; Caseine/caséine/Kasein/caseína; Alpha-S1-casein; Casoxin-D
- UOM
- FOB US$ 1,512.00 US$ 2,520.00 US$ 4,536.00 US$ 8,820.00 US$ 15,120.00
- Quantity
Overview
Properties
- Product No.KSJ333Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transduction
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleMilk.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.068ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Casein Alpha (CSN1). No significant cross-reactivity or interference between Casein Alpha (CSN1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Giveaways
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Citations
- Potential Applications of Milk Fractions and Valorization of Dairy By-Products: A Review of the State-of-the-Art Available Data, Outlining the Innovation Potential from a Bigger Data StandpointJournal: 57876
- Inhibition of arginase via jugular infusion of Nω-hydroxy-nor-l-arginine inhibits casein synthesis in lactating dairy cowsPubmed:29397169
- Arginine Supply Impacts the Expression of Candidate microRNA Controlling Milk Casein Yield in Bovine Mammary TissuePubmed: 32380753