ELISA Kit DIY Materials for Caspase 7 (CASP7)

CMH1; ICE-LAP3; MCH3; Caspase 7 Apoptosis-Related Cysteine Peptidase; Cysteine Aspartate Protease 7; Cysteinyl Aspartate Specific Proteinases 7

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,037.00 US$ 1,728.00 US$ 3,110.00 US$ 6,048.00 US$ 10,368.00
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Overview

Properties

Product No.KSA449Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategoryEnzyme & Kinase Apoptosis

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleTissue homogenates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.156-10ng/mL
Applicable Sensitivity0.054ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Caspase 7 (CASP7). No significant cross-reactivity or interference betweenCaspase 7 (CASP7) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Protective Effects of Morus alba Leaves Extract on Ocular Functions of Pups from Diabetic and Hypercholesterolemic Mother RatsPubMed: 21697998
Analysis of fine structure and biochemical changes of retina during aging of Wistar albino ratsOnlinelibrary: ceo.12123
Aging-related changes of optic nerve of Wistar albino ratsPubmed:Pmc4039266
Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
Inhibition of small HA fragment activity and stimulation of A2APubMed: 25511416
Effects of elevated body temperature on structure and function of cerebrum and cerebellum of Wistar male albino ratsPdf-Files: Issue-4-2015
Role of Pomegranate Juice and Atorvastatin in Ameliorating Spinal Neurotoxicity of Wistar Rats Maternally Fed on Hypercholesterolemic DietDOI: 10.4172/2155-9929.1000316
Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2InhibitorsPubmed: 30191744
Synergistic effects of pomegranate juice and atorvastatin for improving cerebellar structure and function of breast-feeding rats maternally fed on a high cholesterol dietPubmed: 32339653
Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats