ELISA Kit DIY Materials for Catalase (CAT) Homo sapiens (Human) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.124ng/mL
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  • ELISA Kit DIY Materials for Catalase (CAT) Packages (Simulation)
  • ELISA Kit DIY Materials for Catalase (CAT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Materials for Catalase (CAT). No significant cross-reactivity or interference between Materials for Catalase (CAT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic AneurysmAhajournals: source
  • Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activitiesPubMed: 20880383
  • Antioxidant profile of salivary glands in high fat diet-induced insulin resistance ratsPubmed: 24106991
  • Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Pubmed: 24142649
  • Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar ratsSpringer: Source
  • 过氧化氢酶基因 ３８９Ｃ＞Ｔ多态位点在重庆汉族人群中的分布及其与噪音性耳聋的 关联研究Tmmu:Source
  • Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
  • Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
  • Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
  • КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИdocs:225188
  • Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187
  • Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokinesjcrt:5
  • Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects.pubmed:28242484
  • Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2pubmed:28785522
  • Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder.pubmed:28554099
  • Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oilPubmed:29660615
  • Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails10.1080:10807039.2018.1460800
  • Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayPubmed:30116359
  • A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signalingPubmed:30047218
  • Cholecystectomy Patients with High Plasma Level of Catalase Have Significantly Lower Analgesia Requirement: A Prospective Study of Two Different …Pubmed: 30194197
  • Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with CancerPubmed: 30396975
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formationPubmed: 30606939
  • An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cellsPubmed: 30628023
  • Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer PatientsPubmed: 30711961
  • Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A …Pubmed: 30842172
  • MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injuryPubmed: 31115556
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • European Journal of Biomedical AND Pharmaceutical sciences
  • The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and CancerPubmed: 31892574
  • A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 SignalingPubmed: 32135248
  • Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease DevelopmentPubmed: 32751983
  • AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
  • In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
  • Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE …
  • Plasma catalase (CAT) levels in gallstone disease: Cholecystectomy patients with high plasma levels of CAT have significantly lower number of analgesic …
  • Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
  • Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses
  • Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­33689849
  • Mechanisms of Trx2/ASK1-Mediated Mitochondrial Injury in Pemphigus Vulgaris33763469
  • Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­34064937
  • Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats34278476
  • Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis34945751
  • Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer PatientsPubmed:35241533
  • α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat dietPubmed:35569738
  • Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory …Pubmed:35204215

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