ELISA Kit DIY Materials for Beta Catenin (β-catenin) 
CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein
- UOM
- FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
- Quantity
Overview
Properties
- Product No.KSB021Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryTumor immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.25-2000pg/mL
- Applicable Sensitivity13.4pg/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1). No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Versican and its associated molecules: Potential diagnostic markers for multiple myelomaPubmed:25623955
- Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growthPubmed:25639729
- Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive ratsPubMed: 26396666
- Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA.pubmed:28533512
- Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.pubmed:28754618
- Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISAPubmed: 28533512
- Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast CancerDoi: 10.15369/sujms.30.359
- Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney?Pubmed: 30738287
- Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathwayPubmed: 32028241
- Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling …Pubmed: 33179848
- Hydroxytyrosol as a Promising Ally in the Treatment of FibromyalgiaPubmed: 32784915
- Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme33473259
- QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model34564023