ELISA Kit DIY Materials for Cathepsin K (CTSK) Homo sapiens (Human) Assay Kit DIY Materials

CTS-K; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD; Pycnodysostosis; Cathepsin O; Cathepsin X

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity6.3pg/mL
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  • ELISA Kit DIY Materials for Cathepsin K (CTSK) Packages (Simulation)
  • ELISA Kit DIY Materials for Cathepsin K (CTSK) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Cathepsin K (CTSK). No significant cross-reactivity or interference between Cathepsin K (CTSK) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • MRMT-1 rat breast carcinoma cells and models of bone metastases: improvement of an in vitro system to mimic the in vivo condition.PubMed: S0065128112000773
  • Biochemical and clinical correlation of intraplaque neovascularization using contrast-enhanced ultrasound of the carotid arteryPubmed:24534452
  • Drynaria total flavonoids decrease cathepsin K expression in ovariectomized ratsPubmed:25036175
  • In vitro method for the screening and monitoring of estrogen-deficiency osteoporosis by targeting peripheral circulating monocytesPubMed: 26250906
  • Cathepsin K Contributes to Cavitation and Collagen Turnover in Pulmonary TuberculosisPubMed: 26416658
  • Effect of Vitamin D on Peripheral Blood Mononuclear Cells from Patients with Psoriasis Vulgaris and Psoriatic Arthritispmc:PMC4822855
  • TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42Pubmed:29358671
  • Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitisPubmed: 30409937
  • A bioactive exopolysaccharide from marine bacteria Alteromonas sp. PRIM-28 and its role in cell proliferation and wound healing in vitroPubmed: 30851325
  • Biomarkers of Compromised Implant Fixation
  • Compound K induces neurogenesis of neural stem cells in thrombin induced nerve injury through LXRα signaling in micePubmed: 32371156
  • Информационный алгоритм неинвазивной оценки остеорезорбтивного побочного действия глюкокортикостероидов у больных язвенным колитом
  • Особенности остеоиммунологических аспектов остеорезорбции при периимплантите, хроническом пародонтите и раке альвеолярного отростка и …

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