ELISA Kit DIY Materials for Cholesterol (CH)
- UOM
- FOB US$ 1,142.00 US$ 1,904.00 US$ 3,427.00 US$ 6,664.00 US$ 11,424.00
- Quantity
Overview
Properties
- Product No.KSB701Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayHepatologyGastroenterologyNutrition metabolismHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range0.123-10ng/mL
- Applicable Sensitivity0.055ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Cholesterol (CH). No significant cross-reactivity or interference between Cholesterol (CH) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Nuclear phosphatidylinositol 4, 5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcriptionPubmed:29507116
- Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes MellitusPubmed: 33123597
- Immune responses against oxidized LDL as possible targets for prevention of atherosclerosis in systemic lupus erythematosus33857652
- Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity34659432
- GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
- Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MicePubmed:35740008