ELISA Kit DIY Materials for Collagen Type IV (COL4) Homo sapiens (Human) Assay Kit DIY Materials

Type-IV Collagen

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range7.8-500ng/mL
  • Applicable Sensitivity3.1ng/mL
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  • ELISA Kit DIY Materials for Collagen Type IV (COL4) Packages (Simulation)
  • ELISA Kit DIY Materials for Collagen Type IV (COL4) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Collagen Type IV (COL4). No significant cross-reactivity or interference between Collagen Type IV (COL4) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE InhibitionMolMed: 10_11_liu
  • Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidasePubMed: 19019916
  • Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal HyperplasiaScienceDirect: S0022480409001942
  • Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jellyPubmed: 24739658
  • Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells.Pubmed: 24013407
  • Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathyScienceDirect: S0378874114002414
  • Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver FibrosisScienceDirect: S0973688314000073
  • Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
  • Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
  • Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR-1Pubmed:24548186
  • Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesisPubMed: 25617480
  • The Relationship of the Degree of Hepatic Fibrosis with Hyaluronic Acid, Type 4 Collagen, and Procollagen Type 3 N-Terminal Peptide Levels in Patients with Chronic Viral HepatitisOpenview: 8A0B8C97D72C61741371F33508760B75
  • Pathological characterization and morphometric analysis of hepatic lesions in SHRSP5/Dmcr, an experimental non-alcoholic steatohepatitis model, induced by high-fat and high-cholesterol dietdoi:10.1111
  • Clinical significance of serum laminin and type‑IV collagen levels in cutaneous melanoma patientsPubmed:27330797
  • Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.pubmed:27456745
  • Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte‑associated immunoglobulin‑like receptor 110.3892:mmr.2017.6921
  • Phase I and biomarker study of plerixafor and bevacizumab in recurrent high-grade gliomaPubmed:29941486
  • Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced …Pubmed:29620144
  • Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activitiesPubmed: 30530231
  • High amplitude stretching of ATII cells and fibroblasts results in profibrotic effectsPubmed: 31290711
  • Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathyPubmed: 31511328
  • Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungsPubmed: 32146192
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
  • Advanced glycation end-products associate with podocytopathy in type II diabetic patients
  • Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary StudyPubmed:35625637

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