ELISA Kit DIY Materials for Collagen Type X (COL10)
COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain
- UOM
- FOB US$ 1,260.00 US$ 2,100.00 US$ 3,780.00 US$ 7,350.00 US$ 12,600.00
- Quantity
Overview
Properties
- Product No.KSC156Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryMetabolic pathwayDevelopmental scienceNutrition metabolismBone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.118ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Collagen Type X (COL10). No significant cross-reactivity or interference between Collagen Type X (COL10) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor IPubMed: 26123891
- rAAV-mediated overexpression of sox9, TGF-β and IGF-I in minipig bone marrow aspirates to enhance the chondrogenic processes for cartilage repairPubMed: 26583804
- Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture HealingPubmed: 30339839
- Type X Collagen Assays and Methods of Use Thereof