ELISA Kit DIY Materials for Colony Stimulating Factor 1, Macrophage (MCSF)
CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1
- UOM
- FOB US$ 801.00 US$ 1,335.00 US$ 2,403.00 US$ 4,673.00 US$ 8,010.00
- Quantity
Overview
Properties
- Product No.KSA090Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryCytokineInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1,000pg/mL
- Applicable Sensitivity6.5pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Colony Stimulating Factor 1, Macrophage (MCSF). No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage (MCSF) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Microglia-Derived Macrophage Colony Stimulating Factor Promotes Generation of Proinflammatory Cytokines by Astrocytes in the Periventricular White Matter in the Hypoxic Neonatal BrainWiley: source
- miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1pubmed:27107415
- Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal OriginPubmed: 31183654
- Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡33376718