ELISA Kit DIY Materials for Connective Tissue Growth Factor (CTGF) Rattus norvegicus (Rat) Assay Kit DIY Materials

CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity12.2pg/mL
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  • ELISA Kit DIY Materials for Connective Tissue Growth Factor (CTGF) Packages (Simulation)
  • ELISA Kit DIY Materials for Connective Tissue Growth Factor (CTGF) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis modelPubMed: 19824055
  • Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell functionPubMed: PMC2726461
  • Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound HealingPlosone: 0032128
  • Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathyScienceDirect: S0009912012000987
  • Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development?PubMed: S1053249812010121
  • The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS RatsBesjournal: Source
  • Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosisPubMed: 22846210
  • Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosisPubMed: PMC3351780
  • Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
  • Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapyRo-journal: Source
  • Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice ModelPubmed:Pmc4157792
  • Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal modelsPubmed:24464932
  • Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic LineagePubmed:24792956
  • Disruption of collagen homeostasis can reverse established age-related myocardial fibrosisPubmed:25701883
  • Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitisPubMed: 26190501
  • Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitusPubmed:27049870
  • TGF-β and Physiological Root Resorption of Deciduous Teeth.pubmed:28035998
  • Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypubmed:27516211
  • Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRRpubmed:28004175
  • The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidencepubmed:27531356
  • Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseasespubmed:27686729
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR10.1007/s00380-016-0930-y
  • 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.pubmed:28460256
  • Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.pubmed:29120858
  • Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activationPubmed:29107793
  • Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller CellsPubmed:30123889
  • TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretionPubmed: 30426494
  • Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with …Pubmed: 30413105
  • Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin MPubmed: 31249305
  • Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
  • CTGF Attenuates Tendon-Derived Stem/Progenitor Cell AgingPubmed: 31827530
  • Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced NephrotoxicityPubmed: 33212804
  • Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the …Pubmed: 32943074
  • MicroRNAs and ventricular remodeling in aortic stenosisPubmed: 32682570
  • Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction
  • High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth …34455071
  • Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway

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