ELISA Kit DIY Materials for Cortisol (Cor) Pan-species (General) Assay Kit DIY Materials

Hydrocortisone

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, urine and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range68.59-50,000 pg/m
  • Applicable Sensitivity24.58pg/mL
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  • ELISA Kit DIY Materials for Cortisol (Cor) Packages (Simulation)
  • ELISA Kit DIY Materials for Cortisol (Cor) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Cortisol (Cor). No significant cross-reactivity or interference between Cortisol (Cor) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Use of infrared thermography to assess the influence of high environmental temperature on rabbitsScienceDirect: S0034528813001380
  • Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han ChinesePubmed:24124699
  • Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit modelPubmed:25442388
  • Effect of high temperatures on breeding rabbit behaviourPublish:Source
  • Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental studentsIndex.Php: Jbcd
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chipxpls:7393900
  • Fabrication and characterization of SPR chips with the modified bovine serum albuminarticle:10.1007
  • Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactationacve-2017-0006
  • Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine …Pubmed:29540178
  • Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition:
  • Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …
  • Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons
  • A study on certain blood biomarkers of pregnant Cholistani cows.
  • The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot StudyPubmed: 30866503
  • Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexiaPubmed: 32028434
  • Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirusPubmed: 32926999
  • Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition
  • Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense
  • Anti-stress activity of some plants extracts of the North Caucasus flora
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle33722267
  • Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest33960200
  • The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets33435211
  • Stress response after race and endurance training sessions and competitions in Arabian horses33497894
  • Associations of Chinese social face with cortisol level and glucocorticoid receptor gene
  • Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1 …

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