ELISA Kit DIY Materials for Creatine Kinase B (CK-BB) 
CKB; CKBB; CK-B; CK-1; Creatine Kinase, Brain
- UOM
- FOB US$ 1,368.00 US$ 2,280.00 US$ 4,104.00 US$ 7,980.00 US$ 13,680.00
- Quantity
Overview
Properties
- Product No.KSC030Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryEnzyme & KinaseMetabolic pathwayCardiovascular biologyNeuro science
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.78-50ng/mL
- Applicable Sensitivity0.32ng/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Creatine Kinase, Brain (CKB). No significant cross-reactivity or interference between Creatine Kinase, Brain (CKB) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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