ELISA Kit DIY Materials for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Rattus norvegicus (Rat) Assay Kit DIY Materials

NTX-I; NTX1

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range2.47-200ng/mL
  • Applicable Sensitivity0.89ng/mL
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  • ELISA Kit DIY Materials for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Packages (Simulation)
  • ELISA Kit DIY Materials for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Cross Linked N-Telopeptide Of Type I Collagen (NTXI). No significant cross-reactivity or interference between Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacityScienceDirect: S0899900709004717
  • Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca dietScienceDirect: S875632820800776X
  • Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis modelWiley: Source
  • A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular RiskPubMed: PMC3462931
  • Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolismPubmed:25109232
  • An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized MicePubmed:25184758
  • Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis ImperfectaPubMed: 26679066
  • Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
  • Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants.pubmed:27328151
  • Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo …Pubmed:29520826
  • Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis …Pubmed: 30659666
  • AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activitiesPubmed: 31739209
  • Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two CryogelsPubmed: 32517006
  • Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta33506016
  • Effects of icariin on the fracture healing in young and old rats and its mechanism34511043
  • Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway34512780

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