ELISA Kit DIY Materials for Cyanocobalamin (CNCbl)
VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide
- UOM
- FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
- Quantity
Overview
Properties
- Product No.KSA924Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayNutrition metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range123.5-10000pg/mL
- Applicable Sensitivity49.2pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Cyanocobalamin (CNCbl). No significant cross-reactivity or interference between Cyanocobalamin (CNCbl) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.pubmed:29058145
- Selected Risk Nutritional Factors for Chemotherapy-Induced Polyneuropathy.pubmed:28587059
- Lettore di micropiastre multimodale:
- Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study
- Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, EthiopiaPubmed:35464038