ELISA Kit DIY Materials for Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- UOM
- FOB US$ 1,025.00 US$ 1,708.00 US$ 3,074.00 US$ 5,978.00 US$ 10,248.00
- Quantity
Overview
Properties
- Product No.KSJ322Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryImmune molecule
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.119ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Cystatin B (CSTB). No significant cross-reactivity or interference between Cystatin B (CSTB) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disordersPubMed: 23291538
- Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancerPubMed: PMC3754495
- Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal ApoptosisPubMed: PMC3365072
- Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophagesPubmed:25209871
- A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive systemScience: Article
- Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.pubmed:28766361