ELISA Kit DIY Materials for Dihydrotestosterone (DHT) Pan-species (General) Assay Kit DIY Materials

5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range30.9-2500pg/mL
  • Applicable Sensitivity13.3pg/mL
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  • ELISA Kit DIY Materials for Dihydrotestosterone (DHT) Packages (Simulation)
  • ELISA Kit DIY Materials for Dihydrotestosterone (DHT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT). No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar RatsIsrn: 130846
  • Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon ExtractionJofamericanscience: Source
  • Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal modelPubmed:25256687
  • Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2PubMed: 26088557
  • Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARαpubmed:27413362
  • Dihydrotestosterone Treatment Accelerates Autograft Reversal Sciatic Nerve Regeneration in RatsPubmed:29340845
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regressionPubmed: 30671954
  • Plant cell culture extract of Cirsium eriophorum with skin pore refiner activity by modulating sebum production and inflammatory responsePubmed: 32816329
  • Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast CancerPubmed:35323344
  • Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cellsPubmed:35697131

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