ELISA Kit DIY Materials for Dopamine (DA) Pan-species (General) Assay Kit DIY Materials

2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity
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  • ELISA Kit DIY Materials for Dopamine (DA) Packages (Simulation)
  • ELISA Kit DIY Materials for Dopamine (DA) Packages (Simulation)
  • KSA851Ge11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Dopamine (DA). No significant cross-reactivity or interference between Dopamine (DA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis PatientsPubmed: 23530612
  • Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial PerturbationsPubmed:25502462
  • Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological AberrationsPubmed:25526861
  • Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adultsPubmed:25824645
  • Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activityPubMed: 25843409
  • Catha edulis chewing effects on treatment of paranoid schizophrenic patientsPubMed: 25926735
  • Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathwaysPubMed: 25752913
  • Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair testscience:S2314853516300166
  • Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in ratspubmed:27365173
  • Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes.pubmed:27488111
  • Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in ratsS0899900716302350
  • Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996
  • Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Ratsarticle_1486_c0fa9f8afe5f70b407e7195bcfd55c70
  • High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in ratsPubmed:29842916
  • Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor …Pubmed:29854627
  • Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytesPubmed:28948423
  • Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
  • Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring ratsPubmed: 30423388
  • Dopamine levels in human tear fluidPubmed: 30574889
  • Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
  • Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brainPubmed: 31134480
  • Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome
  • Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathwayPubmed: 31740235
  • Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagyPubmed: 31926197
  • Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's diseasePubmed: 31904393
  • Neurotransmitter system aberrations in patients with drug addictionPubmed: 32804296
  • Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemiaPubmed: 32517478
  • Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine
  • Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects
  • The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice33303990
  • Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6?¡­33433498
  • Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­33762821
  • O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
  • A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster34654742
  • Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease34197084
  • Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent ratsPubmed:35141770

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