ELISA Kit DIY Materials for Endostatin (ES)
- UOM
- FOB US$ 1,368.00 US$ 2,280.00 US$ 4,104.00 US$ 7,980.00 US$ 13,680.00
- Quantity
Overview
Properties
- Product No.KSA542Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityCardiovascular biology
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ng/mL
- Applicable Sensitivity0.54ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Endostatin (ES). No significant cross-reactivity or interference between Endostatin (ES) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in miceSpringerLink: u38777121t6660l5
- Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer TherapySpringerLink: 4v306130302257w0
- Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinomaWiley: source
- Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinomaScienceDirect: S075333221100151X
- Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in RatsScialert: Source
- Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast CancerPubMed: PMC3619527
- Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysemaPubmed:24911647
- Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy?Source
- Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndromePubMed: 26125086
- Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the LiteraturePubMed: 26142066
- Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkersscience:S1646706X16300052
- Impaired angiogenesis as a feature of digital ulcers in systemic sclerosisPubmed:26920752
- Blood biomarker for early blood brain barrier disruption in ischemic strokepatent:US20170199205A1