ELISA Kit DIY Materials for Endostatin (ES) Rattus norvegicus (Rat) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ng/mL
  • Applicable Sensitivity0.54ng/mL
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  • ELISA Kit DIY Materials for Endostatin (ES) Packages (Simulation)
  • ELISA Kit DIY Materials for Endostatin (ES) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Endostatin (ES). No significant cross-reactivity or interference between Endostatin (ES) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in miceSpringerLink: u38777121t6660l5
  • Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer TherapySpringerLink: 4v306130302257w0
  • Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinomaWiley: source
  • Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinomaScienceDirect: S075333221100151X
  • Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in RatsScialert: Source
  • Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast CancerPubMed: PMC3619527
  • Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysemaPubmed:24911647
  • Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy?Source
  • Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndromePubMed: 26125086
  • Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the LiteraturePubMed: 26142066
  • Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkersscience:S1646706X16300052
  • Impaired angiogenesis as a feature of digital ulcers in systemic sclerosisPubmed:26920752
  • Blood biomarker for early blood brain barrier disruption in ischemic strokepatent:US20170199205A1

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