ELISA Kit DIY Materials for Endothelin 1 (EDN1) Homo sapiens (Human) Assay Kit DIY Materials

ET1; PPET1; Preproendothelin-1; Big endothelin-1

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range6.17-500pg/mL
  • Applicable Sensitivity2.71pg/mL
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  • ELISA Kit DIY Materials for Endothelin 1 (EDN1) Packages (Simulation)
  • ELISA Kit DIY Materials for Endothelin 1 (EDN1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sicknessAPS: source
  • CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1PubMed: PMC3695508
  • Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic micePubmed: 24212843
  • Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
  • One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneysPubmed:24977654
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioningSciencedirect:S0022480414004909
  • The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafilPubMed: 26023989
  • The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patientsPubMed: 26690031
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injuryPubMed: 26078283
  • Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromescience:article
  • Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients.pubmed:27496444
  • Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X.pubmed:27884380
  • Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactosepdfcopy:42552
  • Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose587e5b6508aed3826af45e2d.pdf
  • Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edemapubmed:27760029
  • Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromearticle:S1872-9312(16)30085-0
  • Biochemical and Molecular Markers of Congenital and Senile Cataractous LensesDOI: 10.4172/2155-9929.1000318
  • A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats.AN=123819331
  • Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation10.1016/j.jff.2017.05.033
  • Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute …Pubmed:29809108
  • Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
  • Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …Pubmed: 30359461
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight …Pubmed: 30570214
  • Whitening effects of cosmetic formulation in the vascular component of skin pigmentationPubmed: 31074159
  • APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan
  • Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive RatsPubmed: 33133215
  • Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
  • Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti …34736077
  • Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells34752899
  • Post-stroke treatment of storax improves long-term outcomes of stroke in rats34343648
  • Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency

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