ELISA Kit DIY Materials for Factor Related Apoptosis (FAS) Mus musculus (Mouse) Assay Kit DIY Materials

CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.25-2000pg/mL
  • Applicable Sensitivity10.2pg/mL
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  • ELISA Kit DIY Materials for Factor Related Apoptosis (FAS) Packages (Simulation)
  • ELISA Kit DIY Materials for Factor Related Apoptosis (FAS) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Factor Related Apoptosis (FAS). No significant cross-reactivity or interference between Factor Related Apoptosis (FAS) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Montelukast abrogates rhabdomyolysis-induced acute renal failure via rectifying detrimental changes in antioxidant profile and systemic cytokines and apoptotic factors production ScienceDirect: S0014299912002580
  • Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat dietWiley: Source
  • The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese womenSpringer:Source
  • The multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosisPubMed: 26269412
  • The heat shock protein 90 inhibitor, 18-AAG, attenuates thioacetamide induced liver fibrosis in miceScience: Article
  • Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation10.1371
  • The Utility of Biomarkers in Osteoporosis Managementpubmed:28271451
  • Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulationpubmed:28081181
  • Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patientspubmed:28655573
  • Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet10.1016/j.jff.2017.07.020
  • Zinc Oxide Nanoparticles Induced Oxidative DNA Damage, Inflammation and Apoptosis in Rat's Brain after Oral ExposurePubmed:29861430
  • The correlation between the level of doxorubicin-induced cardiac damage and serum soluble Fas in an experimental rat modelDoi: 10.4103/ijmpo.ijmpo_82_17
  • EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expressionPubmed: 32703964

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