ELISA Kit DIY Materials for Ferroportin (FPN) Homo sapiens (Human) Assay Kit DIY Materials

SLC40A1; FPN1; HFE4; IREG1; MTP1; SLC11A3; Solute Carrier Family 40,Member 1; Iron-Regulated Transporter

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.134ng/mL
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  • ELISA Kit DIY Materials for Ferroportin (FPN) Packages (Simulation)
  • ELISA Kit DIY Materials for Ferroportin (FPN) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Ferroportin (FPN). No significant cross-reactivity or interference between Ferroportin (FPN) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Host H67D Genotype Affects Tumor Growth in Mouse MelanomaOpen-Access: 1948-5956-1000353
  • The role of HFE genotype in macrophage phenotypePubmed:29391061
  • Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим …
  • COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION
  • Vanadium Decreases Hepcidin mRNA Gene Expression in STZ-Induced Diabetic Rats, Improving the Anemic State33920401
  • Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasisPubmed:35698613
  • Roles of homopolymeric apoferritin in alleviating alcohol-induced liver injury

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