ELISA Kit DIY Materials for Fibrinogen (FG) Homo sapiens (Human) Assay Kit DIY Materials

F1; FI; Fbg; Coagulation Factor I

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range12.50-800ng/mL
  • Applicable Sensitivity5.6ng/mL
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  • ELISA Kit DIY Materials for Fibrinogen (FG) Packages (Simulation)
  • ELISA Kit DIY Materials for Fibrinogen (FG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibrinogen (FG). No significant cross-reactivity or interference between Fibrinogen (FG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • Biomarkers of ketosis in dairy cows at postparturient period: acute phase proteins and pro-inflammatory cytokinesdoi: 10.24099/vet.arhiv.160126c
  • Biomarkeri ketoze u mliječnih krava u postpartalnom razdoblju: proteini akutne faze i proupalni citokinidoi: 10.24099/vet.arhiv.160126c
  • Spike protein up-regulates inflammatory axis of both thromboinflammation and leukotriene in severe COVID-19
  • CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells33421503
  • Influence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactation

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