ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2) Rattus norvegicus (Rat) Assay Kit DIY Materials

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4000pg/mL
  • Applicable Sensitivity
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  • ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2) Packages (Simulation)
  • ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2) Packages (Simulation)
  • KSA551Ra01.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2). No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass modelPubMed: 20438940
  • Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesisPubMed: PMC3254262
  • Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesisTandfonline: 644918
  • Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb IschemiaPubmed: 22266669
  • Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic RatsPubmed: 22091745
  • Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in RatsScialert: Source
  • Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart.PubMed: 22827927
  • Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesisPubMed: PMC3771850
  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesisPubmed: 23952606
  • Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness woundsPubmed: 23934870
  • Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate NeurotoxicityPubmed:25426952
  • Effects of Hemostatic Agents on Fibroblast CellsLww:Source
  • bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healingRsc:Source
  • Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryosPubmed:25530495
  • The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in EwesScience: Article
  • Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosaarticle:S1742706115301410
  • Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scarsPubmed:26774564
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPApubmed:27456338
  • 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts898221
  • FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesispubmed:28841454
  • 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblastspubmed:28052053
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice10.1166/jbt.2017.1652
  • Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular RestorationPubmed:29630447
  • Increased serum FGF2 levels in first-episode, drug-free patients with schizophreniaPubmed: 30172685
  • Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cellsPubmed: 30359541
  • Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and …
  • HOXA9 is a novel myopia risk genePubmed: 30674274
  • Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
  • Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factorPubmed: 31669319
  • β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe DiseasePubmed: 31905906
  • Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditionsPubmed: 32323787
  • Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells
  • New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density33842739
  • Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia33878473
  • Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing33812576
  • Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨¹zerine etkisi
  • Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosisPubmed:35070009
  • Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats

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