ELISA Kit DIY Materials for Folic Acid (FA)
VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate
- UOM
- FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
- Quantity
Overview
Properties
- Product No.KSA610Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryHematologyReproductive scienceNutrition metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range78.1-20,000pg/mL
- Applicable Sensitivity27.5pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Folic Acid (FA). No significant cross-reactivity or interference between Folic Acid (FA) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.pubmed:29058145
- Folic Acid-Functionalized Hybrid Photonic Barcodes for Capture and Release of Circulating Tumor CellsPubmed:29882648
- Development of Orally Applicable, Combinatorial Drug–Loaded Nanoparticles for the Treatment of FibrosarcomaPubmed:29339136
- Role of folic acid deficiency as a possible risk factor for erectile dysfunctionPubmed: 29157083
- Low serum folic acid can be a potential independent risk factor for erectile dysfunction: a prospective case–control studyPubmed: 30547361
- Enhanced cellular uptake, transport and oral bioavailability of optimized folic acid-loaded chitosan nanoparticlesPubmed:35292282
- Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, EthiopiaPubmed:35464038