ELISA Kit DIY Materials for Forkhead Box P3 (FOXP3)

FOX-P3; AIID; DIETER; IPEX; JM2; PIDX; XPID; SCURFIN; Forkhead Box Protein P3; Immune Dysregulation,Polyendocrinopathy,Enteropathy,X-Linked

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
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Overview

Properties

Product No.KSB877Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategorySignal transduction Apoptosis Tumor immunity Infection immunity Immune molecule

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.312-20ng/mL
Applicable Sensitivity0.125ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Forkhead Box Protein P3 (FOXP3). No significant cross-reactivity or interference between Forkhead Box Protein P3 (FOXP3) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Dichloroacetate at therapeutic concentration alters glucose metabolism and induces regulatory T-cell differentiation in alloreactive human lymphocytesPubmed: 23612652
Genetic Polymorphisms of Foxp3 in Patients with Rheumatoid ArthritisPubmed:25448791#
Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patientsarticle:30785
The Role of T-Regulatory Expression in Autoimmune Thyroid Disease and its Association with Thyroid _x005f_x0004_Antibodythe-role-of-tregulatory-expression-in-autoimmune-t
gene polymorphisms and its protein level in patients with rheumatoid arthritis: a case–control studyPubmed:29411043
Therapeutic potential of tranilast for the treatment of chronic graft-versus-host disease in micePubmed: 30307955
Toll-Like Receptor 4–Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Shikonin Treatment of CIA by Regulating Treg/Th17 ExpressionDoi: 10.1155/2018/2428546
Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration ¡33577585