ELISA Kit DIY Materials for Galanin (GAL) Rattus norvegicus (Rat) Assay Kit DIY Materials

GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.97pg/mL
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  • ELISA Kit DIY Materials for Galanin (GAL) Packages (Simulation)
  • ELISA Kit DIY Materials for Galanin (GAL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Galanin (GAL). No significant cross-reactivity or interference between Galanin (GAL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Decreased galanin serum levels are associated with alcohol-craving during withdrawalScienceDirect: S0278584610005191
  • Circulating galanin levels are increased in patients with gestational diabetes mellitusPubMed: 23266294
  • Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus.Pubmed: 24503374
  • Circulating galanin and IL-6 concentrations in gestational diabetes mellitusPubmed:24397394
  • Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitusPubMed: 26172564
  • The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory EpilepsyArticle: 10.1007
  • Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients688687ca8d5a1d03ed060f761328001ca673.pdf
  • Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissuespubmed:27899999
  • Serum Galanin Levels in Young Healthy Lean and Obese Non-Diabetic Men during an OralGlucose Tolerance Test.pubmed:27550417
  • Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
  • Serum Galanin in Children with Autism Spectrum Disorder33534034

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