ELISA Kit DIY Materials for Gamma-Aminobutyric Acid (gABA)
4-Aminobutyric Acid
- UOM
- FOB US$ 1,211.00 US$ 2,019.00 US$ 3,634.00 US$ 7,067.00 US$ 12,114.00
- Quantity
Overview
Properties
- Product No.KSA900Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionCardiovascular biologyNeuro scienceBone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range24.69-2000pg/mL
- Applicable Sensitivity9.27pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Gamma-Aminobutyric Acid (gABA). No significant cross-reactivity or interference between Gamma-Aminobutyric Acid (gABA) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellumPubMed: 21444355
- Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear.PubMed: 18577424
- Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismPubMed: PMC3623607
- Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenPubMed: PMC3694825
- Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
- Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertensionpubmed:27917928
- Sex differences in the glutamate signaling pathway in juvenile ratsPubmed:28861894
- A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzymePubmed: 30341396
- Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of NeurotransmissionPubmed: 30666488
- Differentiation of human glioblastoma U87 cells into cholinergic neuronPubmed: 30928478
- Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism
- Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.
- Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsyPubmed: 33101467
- Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation33828024
- Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases34101060
- Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission34475508
- Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studiesPubmed:35102569