ELISA Kit DIY Materials for Gastric Inhibitory Polypeptide (GIP) Homo sapiens (Human) Assay Kit DIY Materials

Incretin hormone; Glucose Dependent Insulinotropic Peptide

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range61.7-5000pg/mL
  • Applicable Sensitivity23.9pg/mL
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  • ELISA Kit DIY Materials for Gastric Inhibitory Polypeptide (GIP) Packages (Simulation)
  • ELISA Kit DIY Materials for Gastric Inhibitory Polypeptide (GIP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Gastric Inhibitory Polypeptide (GIP). No significant cross-reactivity or interference between Gastric Inhibitory Polypeptide (GIP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus.pubmed:27721485
  • The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats.pubmed:28840471
  • Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague …Pubmed:29098544
  • Predictors of Effectiveness of Glucagon-Like Peptide-1 Receptor Agonist Therapy in Patients with Type 2 Diabetes and Obesity
  • Low AS160 and high SGK basal phosphorylation associates with impaired incretin profile and type 2 diabetes in adipose tissue of obese patientsPubmed: 31734225
  • The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in RatsPubmed: 31701411
  • Prediction scale of response to liraglutide therapy as the method for increase of treatment efficacy in type 2 diabetesPubmed:35251693

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