ELISA Kit DIY Materials for Gastrin (GT) Rattus norvegicus (Rat) Assay Kit DIY Materials

GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.83pg/mL
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  • ELISA Kit DIY Materials for Gastrin (GT) Packages (Simulation)
  • ELISA Kit DIY Materials for Gastrin (GT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Gastrin (GT). No significant cross-reactivity or interference between Gastrin (GT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Omeprazole improves the antiobesity and antidiabetic activity of Exendin-4 in db/db mice.PubMed: 22830490
  • Liver-Derived Systemic Factors Drive b Cell Hyperplasia in Insulin-Resistant StatesPubMed: PMC3655439
  • Lansoprazole enhances the antidiabetic effect of sitagliptin in mice with diet-induced obesity and healthy human subjectsPubmed: 24628303
  • Combination of omeprazole with GLP-1 agonist therapy improves insulin sensitivity and antioxidant activity in liver in type 1 diabetic micePubmed: 24145087
  • Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptideNCBI: PMC4000510
  • 清醒状态下热射病大鼠胃肠动力与胃泌素、胃动素的变化Wjgnet:Source
  • Effects of sphincter of Oddi motility on the formation of cholesterol gallstonesPubmed:27350732
  • Engineering bioinspired bacteria-adhesive clay nanoparticles with a membrane-disruptiveproperty for the treatment of Helicobacter pylori infection.pubmed:27605059
  • The Effect of Salt Intake and Potassium Supplementation on Serum Gastrin Levels in Chinese Adults: A Randomized Trialpubmed:28420122
  • Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation10.1007/s13765-017-0327-3
  • Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric UlcerPubmed: 30479041
  • Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
  • The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion FormationPubmed:35069760

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