ELISA Kit DIY Materials for Gastrin Releasing Peptide (GRP) Rattus norvegicus (Rat) Assay Kit DIY Materials

BN; GRP10; Bombesin; Neuromedin C

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.69pg/mL
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  • ELISA Kit DIY Materials for Gastrin Releasing Peptide (GRP) Packages (Simulation)
  • ELISA Kit DIY Materials for Gastrin Releasing Peptide (GRP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Gastrin Releasing Peptide (GRP). No significant cross-reactivity or interference between Gastrin Releasing Peptide (GRP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

  • Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis.pubmed:27686401

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