ELISA Kit DIY Materials for Gelsolin (GSN) Bos taurus; Bovine (Cattle) Assay Kit DIY Materials

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range7.8-500ng/mL
  • Applicable Sensitivity3.5ng/mL
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  • ELISA Kit DIY Materials for Gelsolin (GSN) Packages (Simulation)
  • ELISA Kit DIY Materials for Gelsolin (GSN) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Gelsolin (GS). No significant cross-reactivity or interference between Gelsolin (GS) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of EpilepsySpringerLink: 3585r8h46q7r0171
  • Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injuryBioMed: 17422094
  • Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancerIEEE: 6098563
  • Investigation of the actin scavenging system in pre-eclampsiaPubmed: 24239294
  • Proteomic Study Reveals Plasma Protein Changes in Congenital Heart DiseasesPubmed: 24565402
  • The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot studyPubmed:Pmc4132929
  • Gelsolin: Role of a Functional Protein in Mitigating Radiation InjuryPubmed:25164111
  • Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer diseasePubMed: 25864780
  • Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
  • Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
  • Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results.pubmed:28365030
  • Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental MethodsPubmed:30040720
  • Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young childrenPubmed:29427164
  • Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various …Pubmed:35327525

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